Unable to connect to database

I'm getting this error when my second register trys to connect to the server When I test the connection under the store adminstrator I get the following response

Error #2147467259 [DBNETLIB]{ConnectionOpen(PreloginHandshake()).]General network error. Check your network documentation.(Source:Microsoft OLE DB Provider for SQL Server) (SQL State: 08001) (NativeError: 11) No Help Files available. This system has been running for over a year before this happend. The physical\ network seem to be fine as we can see files and folders on the server register. Need Help with this one.

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Paul Houston / Eastern National
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GPL Integrated IT

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Paul Houston / Eastern National

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